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The alignment was trimmed using TrimAL (-gappyout) [61] and subject to model selection (WAG+GAMMA with invariable sites) and Bayesian phylogeny construction (Mr Bayes) with two million five hundred thousand generations, a sample frequency of 10%, and a burn in rate of 30% carried out in TOPALi v2.5 [62]

In an absolute pinch, your insulated travel case with a refreshed cold pack can maintain safe temperatures for 12 to 24 hours, giving you time to find proper refrigeration